ABSTRACT
Objective To understand the awareness of schistosomiasis control knowledge among people going to Africa in Taizhou City, to provide insights into the development of control measures for imported schistosomiasis. Methods During the period from July to October, 2019, a face-to-face questionnaire survey was conducted among people that had gone to African countries between 2015 and 2018 and return to China now, including awareness of schistosomiasis control knowledge, type of living and drinking water and contact with river and lake water. Results A total of 573 peoples going to Africa were investigated, and 572 eligible questionnaires were recovered. Among the 572 people interviewed, senior high school and above was the predominant education level (509 people, 89.0%), labor was the predominant aim (543 people, 94.9%), and Angola (52.3%) and Algeria (15.9%) were predominant destiny countries. There were 7.2% of the interviewees hearing about African schistosomiasis, 19.8% knowing infections by contact with infested water, and 36 people with a history of contact with river and lake water. In addition, 270 interviewees used running water as drinking water and 276 used running water as living water. Conclusions The awareness rate of schistosomiasis control knowledge is low among people going to Africa in Taizhou City. Health education pertaining to schistosomiasis control is required to be improved among those going to Africa in Taizhou City to increase the awareness of schistosomiasis prevention and avoid parasitic infections.
ABSTRACT
Objective To analyze a case infected with Plasmodium falciparum due to blood transfusion in Taizhou City, so as to provide scientific evidence for malaria control. Methods The epidemiological investigation of a case with P. falciparum malaria was conducted, and the blood donors’blood samples were collected and detected in laboratory to identify the origin of the infection. Results Microscopy detected Plasmodium in the blood samples from a 64-year-old male inpatient with nephropathy, and P. falciparum malaria was diagnosed. The case had no history of travel to Africa or Southeast Asia except blood transfusion, and he had received blood transfusion 14 times during his hospital stay. Detection of blood donors’blood samples showed P. falciparum in the archived blood samples from an Indonesian student studying in China, as revealed by PCR assay, and the student was found to have a history of malaria. Conclusions The patient was confirmed to be a case with P. falciparum malaria due to blood transfusion. Screening of malaria should be intensified in blood donors to prevent the development of malaria transmitted by blood transfusion.
ABSTRACT
Objective To analyze a case infected with Plasmodium falciparum due to blood transfusion in Taizhou City, so as to provide scientific evidence for malaria control. Methods The epidemiological investigation of a case with P. falciparum malaria was conducted, and the blood donors’blood samples were collected and detected in laboratory to identify the origin of the infection. Results Microscopy detected Plasmodium in the blood samples from a 64-year-old male inpatient with nephropathy, and P. falciparum malaria was diagnosed. The case had no history of travel to Africa or Southeast Asia except blood transfusion, and he had received blood transfusion 14 times during his hospital stay. Detection of blood donors’blood samples showed P. falciparum in the archived blood samples from an Indonesian student studying in China, as revealed by PCR assay, and the student was found to have a history of malaria. Conclusions The patient was confirmed to be a case with P. falciparum malaria due to blood transfusion. Screening of malaria should be intensified in blood donors to prevent the development of malaria transmitted by blood transfusion.
ABSTRACT
<p><b>OBJECTIVE</b>To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.</p><p><b>METHODS</b>The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.</p><p><b>RESULTS</b>pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.</p><p><b>CONCLUSIONS</b>The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.</p>
Subject(s)
Escherichia coli , Genetics , Metabolism , Fluorescent Dyes , Genes, Essential , Genes, Reporter , Genetic Vectors , Luminescent Proteins , Genetics , Operon , Plasmids , Promoter Regions, Genetic , Rec A Recombinases , Genetics , Metabolism , Recombinant Proteins , Genetics , Metabolism , Streptococcus mutans , Genetics , Transformation, BacterialABSTRACT
Objective To report a case of foot hyalohyphomycosis due to Fusarium subglutinans. Methods Medical history was collected and physical examination performed for this patient.Biopsy samples were obtained from the inner side of right ankle of this patient and subjected to pathological examination. Discharge was collected from the lesions for direct microscopic examination and culture.Results A 72-year-old woman presented with an ulcer on the right foot for 3 years.Physical examination disclosed an ulcer,measuring 3 cm x 1.5 cm,with a moist surface and obvious tenderness,at the inner side of the right ankle.Proliferation of dusky-red granulomatous tissue was observed at the base of the ulcer.Pathological examination revealed necrotic granulomatous tissue and slender,septate and hyaline hypha-like structure in the superficial dermis with scattered infiltration of inflammatory cells.PAS staining showed sausage-like hypha and scattered orbicular-ovate spores.Microscopic examination of lesional discharge exhibited septate, branching and hyaline hypha.The isolated fungus was identified as Fusarium subglutinans by culture,and appeared to be highly sensitive to terbinafine,nystatin and amphotericin B.The lesion completely healed after 2 months of treatment with oral terbinafine (0.25 g,twice a day).Conclusions This is a case of foot hyalohyphomycosis due to Fusarium subglutinans,and terbinafine is effective for this condition.